Regulatory

Part:BBa_K4825021:Design

Designed by: Qiu Tianhou   Group: iGEM23_GreatBay-SCIE   (2023-10-12)


pNOTAra1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

""1. AraI is selected while AraO2 is not to explore the oppotunity to design a NOT gate. The arabinose binding condtion of AraC will bind to AraI, which enables us to directly try to use it as a repressor instead of using the original DNA loop regulation. 2.T-rich spacers results in nucleosome-free regions to reduce the potential impacts from neighbouring DNA.

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Source

Synthesized

References

"Robert Schleif. Regulation of the l-arabinose operon of Escherichia coli. Trends in Genetics, Volume 16, Issue 12, 2000, Pages 559-565, ISSN 0168-9525, https://doi.org/10.1016/S0168-9525(00)02153-3.

Schleif R. A Career's Work, the l-Arabinose Operon: How It Functions and How We Learned It. EcoSal Plus. 2022 Dec 15;10(1):eESP00122021. doi: 10.1128/ecosalplus.ESP-0012-2021. Epub 2021 Aug 18. PMID: 36519894."